Determination of methotrexate using carbon paste electrode modified with ionic liquid/Ni-Co layered double hydroxide nanosheets as a voltammetric sensor

Peyman Mohammadzadeh Jahani; Fariba Garkani Nejad; Reza Zaimbashi; Mohammad Reza Aflatoonian; Somayeh Tajik; Hadi Beitollahi

doi:10.5599/admet.2460

Authors

Peyman Mohammadzadeh Jahani School of Medicine, Bam University of Medical Sciences, Bam, Iran https://orcid.org/0000-0001-6483-8986
Fariba Garkani Nejad Environment Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran https://orcid.org/0000-0002-3919-950X
Reza Zaimbashi Environment Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran https://orcid.org/0000-0003-2842-6380
Mohammad Reza Aflatoonian Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran https://orcid.org/0009-0009-8225-4357
Somayeh Tajik Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran https://orcid.org/0000-0002-3919-950X
Hadi Beitollahi Environment Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran https://orcid.org/0000-0002-0669-5216

DOI:

https://doi.org/10.5599/admet.2460

Keywords:

Electrochemical sensor, real sample analysis, cyclic voltammetry, differential pulse voltammetry

Abstract

Background and purpose: Methotrexate (MTX) is a widely used anti-cancer drug, but its overuse can lead to significant side effects. Therefore, it is very vital to design simple and sensitive analytical methods for its determination. Experimental approach: In this work, an electrochemical sensor was prepared based on an ionic liquid (IL)/Ni-Co layered double hydroxide nanosheets (Ni-Co-LDH)-modified carbon paste electrode IL/Ni-Co-LDH/CPE. Cyclic voltammetry, differential pulse voltammetry, and chronoamperometry methods were applied to evaluate the performance of the designed sensor for MTX determination. Key results: The IL/Ni-Co-LDH/CPE sensor exhibits a linear relationship between the peak current of the differential pulse voltammetry and MTX concentrations in the linear dynamic range of 0.02 to 140.0 µM, with a detection limit of 0.006 µM. The IL/Ni-Co-LDH/CPE sensor exhibited relative standard deviation values between 1.7 to 3.7 % for recovery tests on real samples, indicating the precision of the method. Conclusion: The designed sensor with cost-effective and good performance could be valuable for therapeutic drug monitoring and clinical diagnostics.

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