Engineering affibody-based biosensing platforms for cancer biomarker detection

Review paper

Authors

  • Zhafira Fauziah Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Sumedang 45363, Indonesia https://orcid.org/0009-0005-0145-1211
  • Robeth Viktoria Manurung Research Center for Smart Mechatronics, National Research and Innovation Agency (BRIN), KST Samaun Samadikun, Bandung, Indonesia https://orcid.org/0000-0001-8641-2249
  • Yuspian Nur Research Center for Smart Mechatronics, National Research and Innovation Agency (BRIN), KST Samaun Samadikun, Bandung, Indonesia https://orcid.org/0000-0002-8900-0810
  • Dika Apriliana Wulandari Study Center of Sensor and Green Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Bandung 40132, Indonesia https://orcid.org/0009-0003-8361-2116
  • Salma Nur Zakiyyah Study Center of Sensor and Green Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Bandung 40132, Indonesia https://orcid.org/0000-0003-0985-5980
  • Irkham Irkham Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Sumedang 45363, Indonesia and Study Center of Sensor and Green Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Bandung 40132, Indonesia https://orcid.org/0000-0001-9938-2931
  • Yeni Wahyuni Hartati Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Sumedang 45363, Indonesia and Study Center of Sensor and Green Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Bandung 40132, Indonesia https://orcid.org/0000-0003-1463-6352

DOI:

https://doi.org/10.5599/admet.3363

Keywords:

Electrochemical biosensor, optical biosensor, diagnostic

Abstract

Background and purpose: Cancer is one of the most life-threatening diseases and has the highest mortality rate worldwide. Delayed cancer diagnosis remains a major challenge in cancer treatment. Currently, conventional diagnostic methods still have several limitations, resulting in many cancer cases being detected only at advanced stages. Experimental approach: This review was conducted as a literature study of research on the development of affibody-based biosensors for cancer biomarker detection. The review discusses the characteristics of affibodies as bioreceptors, affibody synthesis methods, and their applications in electro­chemical and optical biosensors. Key results: Various studies have shown that affibody-based biosensors exhibit high sensitivity and specificity for detecting cancer biomarkers such as human epidermal growth factor receptor 2, tumour necrosis factor-alpha, epidermal growth factor receptor, carcinoembryonic antigen and alpha-fetoprotein. The integration of affibodies into biosensor platforms enables low detection limits, wide linear ranges and good analytical performance in biological samples. Conclusion: Affibody-based biosensors show great potential as platforms for cancer diagnosis. The use of affibodies as bioreceptors offers several advan­tages over antibodies and aptamers, particularly ease of synthesis, high stability and strong binding affinity.

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11-06-2026

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Vol. 14 (2026)

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Pharmaceutical and biomedical analysis

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Copyright (c) 2026 Zhafira Fauziah, Robeth Viktoria Manurung, Yuspian Nur, Dika Apriliana Wulandari, Salma Nur Zakiyyah, Irkham Irkham, Yeni Wahyuni Hartati

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Engineering affibody-based biosensing platforms for cancer biomarker detection: Review paper. (2026). ADMET and DMPK, 14, Article 3363. https://doi.org/10.5599/admet.3363

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