Interaction between albumin originating from persons with uncontrolled diabetes mellitus type 2 and food antioxidants: Original scientific article

Miloš Šunderić; Dragana Dekanski; Olgica Nedić

doi:10.5599/admet.2892

Authors

Miloš Šunderić Institute for the Application of Nuclear Energy (INEP) University of Belgrade https://orcid.org/0000-0002-0940-9481
Dragana Dekanski Institute for the Application of Nuclear Energy (INEP) University of Belgrade https://orcid.org/0000-0001-8467-4165
Olgica Nedić Institute for the Application of Nuclear Energy (INEP) University of Belgrade https://orcid.org/0000-0003-2042-0056

DOI:

https://doi.org/10.5599/admet.2892

Keywords:

(Dihydro)lipoic acid, glucose metabolism, oleuropein, resveratrol, transport/activity of antioxidants

Abstract

Background and Purpose: Dietary interventions are a cornerstone in the management of diabetes mellitus type 2. The efficiency, however, depends on pharmacokinetic factors, including interaction of food ingredients with plasma proteins. The concept of this study was to investigate binding effects of three pronounced antioxidants present in the Mediterranean diet: resveratrol, (dihydro)lipoic acid and oleuropein, with albumin isolated from persons with diabetes (HbA1c 63±7 mmol/mol, or 7.9±0.6%) and healthy persons, carrying its intrinsic ligands. Experimental approach: Spectrofuorometric analysis, native electrophoresis and immunoblotting were performed with albumin before and after the interaction with antioxidants. Key results: Fluorescence spectra of the protein from two study groups were similar, whereas a spectrum of methylglyoxal-modified albumin (in vitro oxidised) was different. Calculated binding constants were also similar for two study groups for all three ligands. Kinetic fluorescence measurements revealed significantly altered activity of albumin-bound (dihydro)lipoic acid in persons with diabetes compared to healthy individuals, and no significant difference in the activity of resveratrol in expressing antioxidant protection of albumin upon its exposure to oxidative stress. Conclusions: Although the findings should be further validated using other antioxidants and glycated albumin derived from persons stratified according to the severity of a disease, the results have documented that in vitro methylglyoxal-oxidised albumin, routinely employed for diabetes-simulated investigations, was shown not to reflect this pathophysiological condition properly and not to be adequate for the assessment of relevant nutritional/biochemical potential of food antioxidants.

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