Porcine gelatine detection via electrochemical immunosensors utilizing green-synthesized cerium oxide nanoparticles from orange peel

Original scientific paper

Authors

  • Rafa Radithya Swara Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia https://orcid.org/0009-0002-2316-1766
  • Nazwa Alya Zahra Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia https://orcid.org/0009-0006-0326-2358
  • Dea Hasna Tsary Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia https://orcid.org/0009-0004-5521-816X
  • Biyas Aurora Nania Nevada Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia https://orcid.org/0009-0004-9151-2710
  • Elisabeth Nasya Dominika Setyawati Putri Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia https://orcid.org/0009-0003-5050-838X
  • Salma Nur Zakiyyah Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia https://orcid.org/0000-0003-0985-5980
  • Yohanes Susanto Ridwan Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia and Directorate of Laboratory Management, Research Facilities, and Science and Technology Park, National Research and Innovation Agency, Republic of Indonesia, Jakarta 10340, Indonesia https://orcid.org/0000-0002-6631-6142
  • Yeni Wahyuni Hartati Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia and Study Center of Sensor and Green Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Bandung 40132, Indonesia https://orcid.org/0000-0003-1463-6352
  • Irkham Irkham Department Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Jalan Raya Bandung-Sumedang Km 21 Jatinangor, Sumedang 45363, Indonesia and Study Center of Sensor and Green Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Bandung 40132, Indonesia https://orcid.org/0000-0001-9938-2931

DOI:

https://doi.org/10.5599/jese.2698

Keywords:

Food ingredient, electrochemical sensing, screen printed electrode, nanoceria, plant extract

Abstract

Maintaining the safety and proper labelling of food products is vital for communities around the world. One of the primary concerns in food safety is the unintentional presence of porcine derivatives such as gelatine in food products. This issue could affect the labelling accuracy of food products, religious dietary observances, regulatory compliance, and public health. Electrochemical immunosensors address this issue by rapidly, portably, and especially with high sensitivity, detecting porcine gelatine. The electrode was modified with CeO2 nanoparticles (NPs) synthesized via a green method using orange peel extract, which enhanced the immobilization of the bioreceptor on the electrode surface. Orange peel extract contains secondary metabolites that can act as stabilizing agents and prevent agglomeration during the synthesis process. The synthesized CeO2 NPs show a cube morphology, producing an average particle size of 12 nm with 77.31 % crystallinity. The performance of green synthesized CeO2 NPs modified electrode are successful for detecting porcine gelatine in solution and commercialized gelatine product, resulting in a limit of detection of 3.53 ppm. These results show the potential of green-synthesized CeO2 NPs for an electrochemical immunosensor for detecting porcine products.

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Published

31-07-2025

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Vol. 15 No. 5 (2025)

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Bioelectrochemistry

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Copyright (c) 2025 Rafa Radithya Swara, Nazwa Alya Zahra, Dea Hasna Tsary, Biyas Aurora Nania Nevada, Elisabeth Nasya Dominika Setyawati Putri, Salma Nur Zakiyyah , Yohanes Susanto Ridwan, Yeni Wahyuni Hartati, Irkham Irkham

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Porcine gelatine detection via electrochemical immunosensors utilizing green-synthesized cerium oxide nanoparticles from orange peel: Original scientific paper. (2025). Journal of Electrochemical Science and Engineering, 15(5), Article 2698. https://doi.org/10.5599/jese.2698

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