Novel and simple electrochemical sensing platform based on bimetallic nanoparticles/screen-printed carbon electrode for sensitive determination of metronidazole
Original scientific paper
DOI:
https://doi.org/10.5599/jese.3610Keywords:
Nitroimidazole derivatives, Antimicrobial properties, CarcinogenicityAbstract
A straightforward method for determining metronidazole (MNZ) using voltammetry is presented in this paper. This approach was developed by modifying screen-printed carbon electrodes (SPCE) with Co-Ce nanoparticles (CC-SPCE). Compared with unmodified SPCE, cyclic voltammetry showed an improved peak current response (-16 µA) at the surface of CC-SPCE and a clearly defined reduction peak for MNZ at -630 mV. Differential pulse voltammetry was used to quantify MNZ electrochemically. The CC-SPCE responded well to MNZ in ideal circumstances. With a low detection limit of 0.005 µM, the CC-SPCE showed a linear concentration range for MNZ (0.008 to 450.0 µM). Ultimately, the presented sensing platform may offer an alternative approach to pharmaceutical product analysis.
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[1] D. Preisig, F. Varum, R. Bravo, C. Hartig, J. Spleiss, S. Abbes, F. Caobelli, D. Wild, M. Puchkov, J. Huwyler, M. Haschke, Colonic delivery of metronidazole-loaded capsules for local treatment of bacterial infections: A clinical pharmacoscintigraphy study, European Journal of Pharmaceutics and Biopharmaceutics 165 (2021)22-30. https://doi.org/10.1016/j.ejpb.2021.05.002 DOI: https://doi.org/10.1016/j.ejpb.2021.05.002
[2] Á. G. Menezes, F. G. Cardoso, G. B. Galego, L.M. Alves, M. D. Arbo, S. C. Garcia, M. C. Velho, J. Boniatti, R. C. Beck, T. Tasca, Secnidazole and metronidazole in the treatment of Trichomoniasis: Comparative study of the mechanism of action and development of innovative 3D printed intrauterine devices, Microbial Pathogenesis 211 (2025) 108222. https://doi.org/10.1016/j.micpath.2025.108222 DOI: https://doi.org/10.1016/j.micpath.2025.108222
[3] A. Bendesky, D. Menéndez, P. Ostrosky-Wegman, Is metronidazole carcinogenic?, Mutation Research/Reviews in Mutation Research 511 (2002) 133-144. https://doi.org/10.1016/S1383-5742(02)00007-8 DOI: https://doi.org/10.1016/S1383-5742(02)00007-8
[4] S. Ghosh, O. Falyouna, H. Onyeaka, A. Malloum, C. Bornman, S. S. AlKafaas, Z. T. Al-Sharify, S. Ahmadi, M. H. Dehghani, A. H. Mahvi, S. Nasseri, Recent progress on the remediation of metronidazole antibiotic as emerging contaminant from water environments using sustainable adsorbents: A review, Journal of Water Process Engineering 51 (2023) 103405. https://doi.org/10.1016/j.jwpe.2022.103405 DOI: https://doi.org/10.1016/j.jwpe.2022.103405
[5] N. F. Farid, N. S. Abdelwahab, A new HPLC methodology for the analysis of metronidazole and dexibuprofen: Application to pharmacokinetic study and comparative greenness assessment, Microchemical Journal 183 (2022) 108048. https://doi.org/10.1016/j.microc.2022.108048 DOI: https://doi.org/10.1016/j.microc.2022.108048
[6] T. Wang, Y. Zhang, X. Lu, H. Xu, Y. Zhao, C. Qi, J. Mu, M. Wang, W. Zhang, Colorimetric detection of metronidazole in animal-derived food using a silver-coated glass slide aptasensor, Journal of Food Composition and Analysis 148 (2025) 108202. https://doi.org/10.1016/j.jfca.2025.108202 DOI: https://doi.org/10.1016/j.jfca.2025.108202
[7] C. Ho, D. W. Sin, K. M. Wong, H. P. Tang, Determination of dimetridazole and metronidazole in poultry and porcine tissues by gas chromatography–electron capture negative ionization mass spectrometry, Analytica Chimica Acta 530 (2005) 23-31. https://doi.org/10.1016/j.aca.2004.09.004 DOI: https://doi.org/10.1016/j.aca.2004.09.004
[8] D. A. Abdel-Kader, E. Y. Hashem, Spectrophotometric determination of Metronidazole antibacterial drug via oxidation with alkaline potassium permanganate, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 259 (2021) 119858. https://doi.org/10.1016/j.saa.2021.119858 DOI: https://doi.org/10.1016/j.saa.2021.119858
[9] L. Hou, Q. Li, J. Li, D. Yang, Y. Yang, Cu, Fe, B/Cur-CDs with peroxidase-and ascorbic acid oxidase-like catalytic activity for dual-signal fluorescence sensing of metronidazole, Food Chemistry 493 (2025) 145757. https://doi.org/10.1016/j.foodchem.2025.145757 DOI: https://doi.org/10.1016/j.foodchem.2025.145757
[10] Y. Orooji, M. H. Irani-Nezhad, R. Hassandoost, A. Khataee, S. R. Pouran, S. W. Joo, Cerium doped magnetite nanoparticles for highly sensitive detection of metronidazole via chemiluminescence assay, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 234 (2020) 118272. https://doi.org/10.1016/j.saa.2020.118272 DOI: https://doi.org/10.1016/j.saa.2020.118272
[11] W. Jin, W. Li, Q. Xu, Q. Dong, Quantitative assay of metronidazole by capillary zone electrophoresis with amperometric detection at a gold microelectrode, ELECTROPHORESIS: An International Journal 21 (2000) 1409-1414. https://doi.org/10.1002/(SICI)1522-2683(20000401)21:7<1409::AID-ELPS1409>3.0.CO;2-0 DOI: https://doi.org/10.1002/(SICI)1522-2683(20000401)21:7<1409::AID-ELPS1409>3.0.CO;2-0
[12] E. Delnavaz, K. Asadpour-Zeynali, Cobalt oxide nanoparticles electrodeposited on glassy carbon electrode for metronidazole determination, Results in Chemistry 7 (2024) 101321. https://doi.org/10.1016/j.rechem.2024.101321 DOI: https://doi.org/10.1016/j.rechem.2024.101321
[13] Y. Y. Li, F. Guo, J. Yang, J. F. Ma, Efficient detection of metronidazole by a glassy carbon electrode modified with a composite of a cyclotriveratrylene-based metal–organic framework and multi-walled carbon nanotubes, Food Chemistry 425 (2023) 136482. https://doi.org/10.1016/j.foodchem.2023.136482 DOI: https://doi.org/10.1016/j.foodchem.2023.136482
[14] E. M. Materón, A. Wong, T. A. Freitas, R. C. Faria, O. N. Oliveira Jr, A sensitive electrochemical detection of metronidazole in synthetic serum and urine samples using low-cost screen-printed electrodes modified with reduced graphene oxide and C60, Journal of Pharmaceutical Analysis 11 (2021) 646-652. https://doi.org/10.1016/j.jpha.2021.03.004 DOI: https://doi.org/10.1016/j.jpha.2021.03.004
[15] B. Muthukutty, B. Arumugam, S. M. Chen, S. K. Ramaraj, Low potential detection of antiprotozoal drug metronidazole with aid of novel dysprosium vanadate incorporated oxidized carbon nanofiber modified disposable screen-printed electrode, Journal of Hazardous Materials 407 (2021) 124745. https://doi.org/10.1016/j.jhazmat.2020.124745 DOI: https://doi.org/10.1016/j.jhazmat.2020.124745
[16] P. Sundaresan, T. W. Chen, S. M. Chen, T. W. Tseng, X. Liu, Electrochemical activation of screen printed carbon electrode for the determination of antibiotic drug metronidazole, International Journal of Electrochemical Science 13 (2018) 1441-1451. https://doi.org/10.20964/2018.02.05 DOI: https://doi.org/10.20964/2018.02.05
[17] L. P. Gauly, M. Coulibaly, S. B. Soro, S. R. N’Dri, A. Trokourey, Differential pulse anodic stripping voltammetric determination of Hg (II) in water at a sodium-kaolin carbon paste electrode based on ion-exchange-assisted preconcentration, Results in Chemistry 29 (2026) 103685. https://doi.org/10.1016/j.rechem.2026.103685 DOI: https://doi.org/10.1016/j.rechem.2026.103685
[18] G. Martínez-García, J. Ganan, D. Pérez-Quintanilla, S. Morante-Zarcero, I. Sierra, Development of a carbon paste electrode modified with a nanocomposite of mercaptopyrimidine-functionalized HMS silica and gold nanoparticles for total atropine and scopolamine determination in plant-based food supplements, Microchemical Journal 227 (2026) 118604. https://doi.org/10.1016/j.microc.2026.118604 DOI: https://doi.org/10.1016/j.microc.2026.118604
[19] G. Siciliano, A. Alsadig, M. S. Chiriacò, A. Turco, A. Foscarini, F. Ferrara, G. Gigli, E. Primiceri, Beyond traditional biosensors: recent advances in gold nanoparticles modified electrodes for biosensing applications, Talanta 268 (2024) 125280. https://doi.org/10.1016/j.talanta.2023.125280 DOI: https://doi.org/10.1016/j.talanta.2023.125280
[20] S. Sahoo, T. L. Dora, N. Jain, S. Pande, T. Roy, R. R. Mishra, Microwave-assisted rapid synthesis of hierarchical porous cobalt oxide nanoparticles as electrode materials for high-performance pseudocapacitors, Journal of Energy Storage 178 (2026) 123715. https://doi.org/10.1016/j.est.2026.123715 DOI: https://doi.org/10.1016/j.est.2026.123715
[21] R. S. Bhat, S. S. Hegde, B. R. Bhat, Electrochemical determination of ascorbic acid using carbon paste electrode modified with cobalt oxide nanoparticles, Electrochimica Acta 542 (2025) 147526. https://doi.org/10.1016/j.electacta.2025.147526 DOI: https://doi.org/10.1016/j.electacta.2025.147526
[22] V. Muthiah, V. Dharuman, Pharmaceutical pollutant ornidazole sensing at mesoporous spinel cobalt cerium oxide and graphene nanoplatelets modified electrode in environmental water and fish samples, Microchemical Journal 223 (2026) 117311. https://doi.org/10.1016/j.microc.2026.117311 DOI: https://doi.org/10.1016/j.microc.2026.117311
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Copyright (c) 2026 Shurooq Abdulfattah Hasan, Ala’a R. Shaker, Hassan Youssef Hussein, Mustafa Mudhafar, Hasan Ali Alsailawi
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