Synthesis of silymarin−selenium nanoparticle conjugate and examination of its biological activity in vitro

  • Sergey Staroverov Saratov State Agrarian University, Saratov, Russian federation
  • Sergey Kozlov Saratov State Agrarian University, Saratov https://orcid.org/0000-0003-2164-8140
  • Alexander Fomin Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russian Federation
  • Konstantin Gabalov Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russian Federation
  • Vitaliy Khanadeev Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russian Federation https://orcid.org/0000-0003-3404-5402
  • Dmitry Soldatov Saratov State Agrarian University, Saratov https://orcid.org/0000-0003-2296-5770
  • Ivan Domnitsky Saratov State Agrarian University, Saratov https://orcid.org/0000-0002-6530-7295
  • Lev Dykman Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russian Federation https://orcid.org/0000-0003-2440-6761
  • Sergey V. Akchurin Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Moscow, Russian Federation
  • Olga Guliy Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russian Federation https://orcid.org/0000-0002-0541-0020
Silymarin-selenium nanoparticle conjugates

Abstract

Silymarin (Sil) was conjugated to selenium nanoparticles (SeNPs) to increase Sil bioavailability. The conjugates were monodisperse; the average diameter of the native SeNPs was ~ 20-50 ± 1.5 nm, whereas that of the conjugates was 30-50 ± 0.5 nm. The use of SeNPs to increase the bioavailability of Syl was examined with the MH-22a, EPNT-5, HeLa, Hep-2, and SPEV-2 cell lines. The EPNT-5 (glioblastoma) cells were the most sensitive to the conjugates compared to the conjugate-free control. The conjugates increased the activity of cellular dehydrogenases and promoted the penetration of Sil into the intracellular space. Possibly, SeNPs play the main part in Sil penetration of cells and Sil penetration is not associated with phagocytosis. Thus, SeNPs are promising for use as a Sil carrier and as protective antigens.

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Published
14-11-2021
Section
Original Scientific Articles