Use of Azospirillum baldaniorum cells in quercetin detection

Matvey Kanevskiy; Irina Kosheleva; Vladislav Menukhov; Elizaveta Zhdanova; Svetlana Borisova; Gennadiy Burygin; Svetlana Konnova; Viktor Bunin; Olga Guliy

doi:10.5599/admet.1661

Authors

Matvey Kanevskiy Chernyshevsky Saratov State University, Saratov 410012, Russia https://orcid.org/0000-0002-5932-6748
Irina Kosheleva Chernyshevsky Saratov State University, Saratov 410012, Russia https://orcid.org/0000-0003-1992-5305
Vladislav Menukhov Chernyshevsky Saratov State University, Saratov 410012, Russia https://orcid.org/0000-0002-8432-0890
Elizaveta Zhdanova Chernyshevsky Saratov State University, Saratov 410012, Russia https://orcid.org/0000-0002-2390-8463
Svetlana Borisova Chernyshevsky Saratov State University, Saratov 410012, Russia
Gennadiy Burygin Chernyshevsky Saratov State University, Saratov 410012, Russia and Institute of Biochemistry and Physiology of Plants and Microorganisms – Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Center of the Russian Academy of Sciences (IBPPM RAS), Saratov 410049, Russia https://orcid.org/0000-0001-8031-9641
Svetlana Konnova Chernyshevsky Saratov State University, Saratov 410012, Russia and Institute of Biochemistry and Physiology of Plants and Microorganisms – Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Center of the Russian Academy of Sciences (IBPPM RAS), Saratov 410049, Russia https://orcid.org/0000-0002-9607-8173
Viktor Bunin EloSystems GbR, Germany https://orcid.org/0000-0001-7685-0931
Olga Guliy Chernyshevsky Saratov State University, Saratov 410012, Russia https://orcid.org/0000-0002-0541-0020

DOI:

https://doi.org/10.5599/admet.1661

Keywords:

Flavonoids, quercetin, Azospirillum, electro-optical analysis, impedance

Abstract

The possibility of detection and determination of flavonoids by using microbial cells was shown for the first time using the quercetin - Azospirillum baldaniorum Sp245 model system. The activity of the flavonoids quercetin, rutin and naringenin toward A. baldaniorum Sp245 was evaluated. It was found that when the quercetin concentration ranged from 50 to 100 µM, the number of bacterial cells decreased. Rutin and naringenin did not affect bacterial numbers. Quercetin at 100 μM increased bacterial impedance by 60 %. Under the effect of quercetin, the magnitude of the electro-optical signal from cells decreased by 75 %, as compared with the no-quercetin control. Our data show the possibility of developing sensor-based systems for the detection and determination of flavonoids.

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