Electrochemical genosensor for detection of a mutation in the adenomatous polyposis coli gene (APC 1306)

Original scientific paper

Authors

  • Lizbeth Espinosa Garcia Laboratorio de Nanotecnología e Ingeniería Molecular, Área Electroquímica, Departamento de Química, CBI, Universidad Autónoma Metropolitana-Iztapalapa (UAM-I), Av. San Rafael Atlixco 186, Iztapalapa, CP 09340 Ciudad de México, Mexico and Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Avenida Wilfrido Massieu s/n, Col. Zacatenco, Del. Gustavo A. Madero, CP 07738 Ciudad de México, Mexico https://orcid.org/0000-0002-7577-5594
  • David García García Laboratorio de Nanotecnología e Ingeniería Molecular, Área Electroquímica, Departamento de Química, CBI, Universidad Autónoma Metropolitana-Iztapalapa (UAM-I), Av. San Rafael Atlixco 186, Iztapalapa, CP 09340 Ciudad de México, Mexico and Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Avenida Wilfrido Massieu s/n, Col. Zacatenco, Del. Gustavo A. Madero, CP 07738 Ciudad de México, Mexico https://orcid.org/0009-0005-3591-6267
  • Eduardo O. Madrigal-Santillán Laboratorio de Medicina de Conservación, Escuela Superior de Medicina, Instituto Politécnico Nacional, “Unidad Casco de Santo Tomás”, Plan de San Luis y Díaz Mirón, Ciudad de México, CP 11340, Mexico https://orcid.org/0000-0003-2264-4598
  • Eduardo Madrigal-Bujaidar Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Avenida Wilfrido Massieu s/n, Col. Zacatenco, Del. Gustavo A. Madero, CP 07738 Ciudad de México, Mexico https://orcid.org/0000-0003-4256-1749
  • Isela Álvarez-González Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Avenida Wilfrido Massieu s/n, Col. Zacatenco, Del. Gustavo A. Madero, CP 07738 Ciudad de México, Mexico https://orcid.org/0000-0003-4011-9121
  • Javier Esteban Jiménez-Salazar Laboratorio de Endocrinología Molecular del Cáncer, Departamento de Biología de la Reproducción, CBS, Universidad Autónoma Metropolitana-Iztapalapa (UAM-I), Av. San Rafael Atlixco 186, Iztapalapa, CP 09340 Ciudad de México, Mexico https://orcid.org/0000-0003-1421-3720
  • Pablo Gustavo Damián Matsumura Laboratorio de Endocrinología Molecular del Cáncer, Departamento de Biología de la Reproducción, CBS, Universidad Autónoma Metropolitana-Iztapalapa (UAM-I), Av. San Rafael Atlixco 186, Iztapalapa, CP 09340 Ciudad de México, Mexico https://orcid.org/0000-0003-4815-2516
  • José Alberto García-Melo Universidad Tecnológica de Tula – Tepeji, Av. Universidad Tecnológica No. 1000, 42830, El Carmen, Tula de Allende, Hidalgo, Mexico https://orcid.org/0000-0003-0340-0272
  • Berenice Carbajal-López Instituto Nacional de Cancerología, Tlalpan 14080, México https://orcid.org/0000-0002-9821-6488
  • Jossimar Coronel-Hernández Instituto Nacional de Cancerología, Tlalpan 14080, México https://orcid.org/0000-0001-6543-5310
  • Carlos Pérez-Plasencia Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México (UNAM), Tlalnepantla 54090, Mexico https://orcid.org/0000-0002-8593-8211
  • Miguel Morales-Rodríguez Departamento de Procesos Productivos, División de Ciencias Básicas e Ingeniería, Universidad Autónoma Metropolitana- Lerma, C.P. 52005, Estado de México, México https://orcid.org/0000-0003-1600-4914
  • Nikola Batina Laboratorio de Nanotecnología e Ingeniería Molecular, Área Electroquímica, Departamento de Química, CBI, Universidad Autónoma Metropolitana-Iztapalapa (UAM-I), Av. San Rafael Atlixco 186, Iztapalapa, CP 09340 Ciudad de México, Mexico and Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Avenida Wilfrido Massieu s/n, Col. Zacatenco, Del. Gustavo A. Madero, CP 07738 Ciudad de México, Mexico https://orcid.org/0000-0001-8217-8563
  • Luis Fernando Garcia-Melo Laboratorio de Nanotecnología e Ingeniería Molecular, Área Electroquímica, Departamento de Química, CBI, Universidad Autónoma Metropolitana-Iztapalapa (UAM-I), Av. San Rafael Atlixco 186, Iztapalapa, CP 09340 Ciudad de México, Mexico https://orcid.org/0000-0001-9477-4178

DOI:

https://doi.org/10.5599/jese.3109

Keywords:

DNA biosensor, DNA mutation, differential pulse voltammetry, cyclic voltammetry, colorectal cancer

Abstract

Colorectal cancer is a type of disease that arises from alterations in the genome, making it challenging to diagnose at an early stage. The difficulty in early detection can com­plicate treatment and lower survival rates. These genomic alterations result in mutations in critical genes, including adenomatous polyposis coli (APC). Some of these mutations, including APC 1306, have been identified as potential biomarkers for early cancer diagnosis. Genosensors offer a promising alternative to these detection methods; however, it is essential to optimize their characteristics, including sensitivity, stability, and reaction conditions, prior to use. In this study, an electrochemical genosensor was deve­loped to detect a specific mutation using voltammetric techniques. The limit of detec­tion was 8.56 pM for cyclic voltammetry and 29.76 pM for differential pulse voltam­metry. The optimal incubation and hybridi­zation temperature were determined to be 55 °C. The geno­sensor remained stable for 14 days, after which its performance gradually declined. The tested electrochemical detection methods demonstrate excellent performance for this type of genosensor, particularly in the presence of doxorubicin. This approach can also be utilized to identify various genetic alterations associated with other chronic degenerative diseases.

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03-03-2026

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Vol. 16 (2026)

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Bioelectrochemistry

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Copyright (c) 2026 Lizbeth Espinosa Garcia, David García García, Eduardo O. Madrigal-Santillán, Eduardo Madrigal-Bujaidar, Isela Álvarez-González, Javier Esteban Jiménez-Salazar, Pablo Gustavo Damián Matsumura, José Alberto García-Melo, Berenice Carbajal-López, Jossimar Coronel-Hernández, Carlos Pérez-Plasencia, Miguel Morales-Rodríguez, Nikola Batina, Luis Fernando Garcia-Melo

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Electrochemical genosensor for detection of a mutation in the adenomatous polyposis coli gene (APC 1306): Original scientific paper. (2026). Journal of Electrochemical Science and Engineering, 16, Article 3109. https://doi.org/10.5599/jese.3109

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