Human Serum Albumin Binding of 2-[(Carboxymethyl)sulfanyl]-4-oxo-4-(4-tert-butylphenyl)butanoic Acid and its Mono-Me Ester

Authors

  • Ilija N Cvijetić Innovation Center of the Faculty of Chemistry, University of Belgrade
  • DuÅ¡an D Petrović Faculty of Chemistry, University of Belgrade
  • Tatjana Ž Verbić Faculty of Chemistry, University of Belgrade
  • Ivan O Juranić Department of Chemistry-IChTM, University of Belgrade
  • Branko J Drakulić Department of Chemistry-IChTM, University of Belgrade

DOI:

https://doi.org/10.5599/admet.2.2.28

Abstract

Interactions of 2-[(carboxymethyl)sulfanyl]-4-oxo-4-(4-tert-butylphenyl)butanoic acid (compound 1) and its mono-Me ester (compound 2) with the human serum albumin (HSA) have been studied by fluorescence spectroscopy. Comp. 1 exerts antiproliferative activity toward human tumor cells and significant selectivity (tumor vs. healthy cells) in vitro. Competitive binding study with warfarin and ibuprofen as binding site probes, revealed that one molecule of comp. 1 selectively binds to HSA Sudlow site I (warfarin site) with moderate binding constant (Kb = (2.8 ± 0.5) ´ 104 M-1 at 37 ± 1 °C), while comp. 2 binds to Sudlow site II (Kb = (3.2 ± 0.9) ´ 104 M-1 at 37 ± 1 °C). Fluorescence quenching at different temperatures was analyzed using classical Stern-Volmer equation, and a static quenching mechanism was proposed. Energy resonance transfer between HSA and comp. 1 was examined according to Förster’s non-radiative energy transfer theory. Distance of about 10 Å between ligand and Trp214 (HSA) was obtained. Docking studies confirmed HSA Sudlow site I as a preferable comp. 1 binding site, and Sudlow site II as comp. 2 binding site. Molecular dynamics simulations proved the stability of comp. 1/HSA complex.

Downloads

Download data is not yet available.

Downloads

Published

18-07-2014

How to Cite

Cvijetić, I. N., Petrović, D. D., Verbić, T. Ž, Juranić, I. O., & Drakulić, B. J. (2014). Human Serum Albumin Binding of 2-[(Carboxymethyl)sulfanyl]-4-oxo-4-(4-tert-butylphenyl)butanoic Acid and its Mono-Me Ester. ADMET and DMPK, 2(2), 126–142. https://doi.org/10.5599/admet.2.2.28

Issue

Section

Original Scientific Articles