Evaluation of curcumin-loaded chitosan nanoparticles for wound healing activity
DOI:
https://doi.org/10.5599/admet.1897Keywords:
Skin regeneration, turmeric, ionotropic gelation, chitosan, nanoparticles, topical administration
Abstract
Background and purpose: Wound healing is a biological process that can be difficult to manage clinically. In skin wound healing, the interaction of many cells, growth factors, and cytokines reveals an outstanding biological function mechanism. Wound healing that occurs naturally restores tissue integrity, however, it is usually restricted to wound repair. Curcumin synthesised in a chitosan matrix can be used to heal skin sores. Experimental approach: The ionotropic gelation procedure required crosslinking chitosan with a tripolyphosphate (TPP) crosslinker to generate curcumin nanoparticles encapsulated in chitosan. Key results: The nanoparticles were between 200 and 400 nm in size, with a strong positive surface charge and good entrapment efficacy, according to SEM and TEM investigations. Curcumin and chitosan compatibility was investigated using FTIR spectroscopy. All batches showed consistent drug release, with the F5 batch having the highest curcumin release, at 75% after 16 hours. On L929 cells, scratch assays were utilised to assess wound healing. Wound closure with widths of 59 and 65 mm with curcumin and 45 and 78 mm with curcumin-loaded chitosan nanoparticles was seen after 24 and 48 hours of examination. Conclusions: According to the findings, prepared curcumin chitosan nanoparticles are beneficial in healing skin damage.
Downloads
References
P.G. Bowler, B.I. Duerden, D.G. Armstrong. Wound microbiology and associated approaches to wound management. Clinical Microbiology Reviews 14 (2001) 244-269. https://doi.org/10.1128/CMR.14.2.244-269.2001
N.I.M. Fadilah, S.J. Phang, N. Kamaruzaman, A. Salleh, M. Zawani, A. Sanyal, M. Maarof, M.B. Fauzi. Antioxidant Biomaterials in Cutaneous Wound Healing and Tissue Regeneration: A Critical Review. Antioxidants (Basel) 12 (2023) 787. https://doi.org/10.3390/antiox12040787
M. Rodrigues, N. Kosaric, C.A. Bonham, G. C. Gurtner. Wound Healing: A Cellular Perspective. Physiological Reviews 99 (2019) 665-706. https://doi.org/10.1152/physrev.00067.2017
P. Krzyszczyk, R. Schloss, A. Palmer, F. Berthiaume. The Role of Macrophages in Acute and Chronic Wound Healing and Interventions to Promote Pro-wound Healing Phenotypes. Frontiers in Physiology 9 (2018) 419. https://doi.org/10.3389/fphys.2018.00419
G.M.A. Ndong Ntoutoume, R. Granet, J.P. Mbakidi, F. Brégier, D.Y. Léger, C. Fidanzi-Dugas, V. Lequart, N. Joly, B. Liagre, V. Chaleix, V. Sol. Development of curcumin-cyclodextrin/cellulose nanocrystals complexes: New anticancer drug delivery systems. Bioorganic & Medicinal Chemistry Letters 26 (2016) 941-945. https://doi.org/10.1016/j.bmcl.2015.12.060
Y.H. Yen, C.M. Pu, C.W. Liu, Y.C. Chen, Y.C. Chen, C.J. Liang, J.H. Hsieh, H.F. Huang, Y.L. Chen. Curcumin accelerates cutaneous wound healing via multiple biological actions: The involvement of TNF-α, MMP-9, α-SMA, and collagen. International Wound Journal 15 (2018) 605-617. https://doi.org/10.1111/iwj.12904
Y. Manoharan, V. Haridas, K.C. Vasanthakumar, S. Muthu, F.F. Thavoorullah, P. Shetty. Curcumin: a Wonder Drug as a Preventive Measure for COVID19 Management. Indian Journal of Clinical Biochemistry 35 (2020) 373-375. https://doi.org/10.1007/s12291-020-00902-9
E. Afzali, T. Eslaminejad, S.E. Yazdi Rouholamini, M. Shahrokhi-Farjah, M. Ansari. Cytotoxicity Effects of Curcumin Loaded on Chitosan Alginate Nanospheres on the KMBC-10 Spheroids Cell Line. International Journal of Nanomedicine 16 (2021) 579-589. https://doi.org/10.2147/IJN.S251056
G. Emiroglu, Z. Ozergin Coskun, Y. Kalkan, O. Celebi Erdivanli, L. Tumkaya, S. Terzi, A. Özgür, M. Demirci, E. Dursun. The Effects of Curcumin on Wound Healing in a Rat Model of Nasal Mucosal Trauma. Evidence-based Complementary and Alternative Medicine 2017 (2017) 9452392. https://doi.org/10.1155/2017/9452392
N. Ibrahim, S.K. Wong, I.N. Mohamed, N. Mohamed, K.Y. Chin, S. Ima-Nirwana, A.N. Shuid. Wound Healing Properties of Selected Natural Products. International Journal of Environmental Research And Public Health 15 (2018) 2360. https://doi.org/10.3390/ijerph15112360
A. Popat, S. Karmakar, S. Jambhrunkar, C. Xu, C. Yu. Curcumin-cyclodextrin encapsulated chitosan nanoconjugates with enhanced solubility and cell cytotoxicity. Colloids and Surfaces. B 117 (2014) 520-527. https://doi.org/10.1016/j.colsurfb.2014.03.005
D. Zhao, S. Yu, B. Sun, S. Gao, S. Guo, K. Zhao. Biomedical Applications of Chitosan and Its Derivative Nanoparticles. Polymers (Basel) 10 (2018) 462. https://doi.org/10.3390/polym10040462
R. de Sousa Victor, A. Marcelo da Cunha Santos, B. Viana de Sousa, G. de Araújo Neves, L. Navarro de Lima Santana, R. Rodrigues Menezes. A Review on Chitosan's Uses as Biomaterial: Tissue Engineering, Drug Delivery Systems and Cancer Treatment. Materials (Basel) 13 (2020) 4995. https://doi.org/10.3390/ma13214995
P. Mohite, S.R. Shah, S. Singh, T. Rajput, S. Munde, N. Ade, B.G. Prajapati, H. Paliwal, D.D. Mori, A.V. Dudhrejiya. Chitosan and chito-oligosaccharide: a versatile biopolymer with endless grafting possibilities for multifarious applications. Frontiers in Bioengineering and Biotechnology 11 (2023) 1190879. https://doi.org/10.3389/fbioe.2023.1190879
H.M. Basit, M.C.I. Mohd Amin, S.F. Ng, H. Katas, S.U. Shah, N.R. Khan. Formulation and Evaluation of Microwave-Modified Chitosan-Curcumin Nanoparticles-A Promising Nanomaterials Platform for Skin Tissue Regeneration Applications Following Burn Wounds. Polymers (Basel) 12 (2020) 2608. https://doi.org/10.3390/polym12112608
W.H. Tsai, K.H. Yu, Y.C. Huang, C.I. Lee. EGFR-targeted photodynamic therapy by curcumin-encapsulated chitosan/TPP nanoparticles. International Journal of Nanomedicine 13 (2018) 903-916. https://doi.org/10.2147/IJN.S148305
A. Isvoran, A.A. Ciorsac, V. Ostafe. ADME-Tox profiling of some low molecular weight water soluble chitosan derivatives. ADMET and DMPK 5(3) (2017) 192-200. https://doi.org/10.5599/admet.5.3.423
P. Sacco, S. Pedroso-Santana, Y. Kumar, N. Joly, P. Martin, P. Bocchetta. Ionotropic Gelation of Chitosan Flat Structures and Potential Applications. Molecules 26 (2021) 660. https://doi.org/10.3390/molecules26030660
W. Zhang, W. Xu, Y. Lan, X. He, K. Liu, Y. Liang. Antitumor effect of hyaluronic-acid-modified chitosan nanoparticles loaded with siRNA for targeted therapy for non-small cell lung cancer. International Journal of Nanomedicine 14 (2019) 5287-5301. https://doi.org/10.2147/IJN.S203113
M. Qi, X. Zhu, X. Yu, M. Ai, W. Cai, B. Du, B. Hou, L. Qiu. Preparation of W/O Hypaphorine-Chitosan Nanoparticles and Its Application on Promoting Chronic Wound Healing via Alleviating Inflammation Block. Nanomaterials (Basel) 11 (2021) 2830. https://doi.org/10.3390/nano11112830
R. Meng, Z. Wu, Q.T. Xie, J.S. Cheng, B. Zhang. Preparation and characterization of zein/car-boxymethyl dextrin nanoparticles to encapsulate curcumin: Physicochemical stability, antioxidant activity and controlled release properties. Food Chemistry 340 (2021) 127893. https://doi.org/10.1016/j.foodchem.2020.127893
A. Enumo, D.F. Argenta, G.C. Bazzo, T. Caon, H.K. Stulzer, A.L. Parize. Development of curcumin-loaded chitosan/pluronic membranes for wound healing applications. International Journal of Biological Macromolecules 163 (2020) 167-179. https://doi.org/10.1016/j.ijbiomac.2020.06.253
R. Shanmugam, R. Subramaniam, S.G. Kathirason, D. Ali, S.R. Balusamy, A. Gurusamy, K. Arunachalam, H. Sellami. Curcumin-Chitosan Nanocomposite Formulation Containing Pongamia pinnata-Mediated Silver Nanoparticles, Wound Pathogen Control, and Anti-Inflammatory Potential. BioMed Research International 2021 (2021) 3091587. https://doi.org/10.1155/2021/3091587
D.J. Pochapski, C. Carvalho Dos Santos, G.W. Leite, S.H. Pulcinelli, C.V. Santilli. Zeta Potential and Colloidal Stability Predictions for Inorganic Nanoparticle Dispersions: Effects of Experimental Conditions and Electrokinetic Models on the Interpretation of Results. Langmuir 37 (2021) 13379-13389. https://doi.org/10.1021/acs.langmuir.1c02056
S. Vogt, K. Löffler, A.G. Dinkelacker, B. Bader, I.B. Autenrieth, S. Peter, J. Liese. Fourier-Transform Infrared (FTIR) Spectroscopy for Typing of Clinical Enterobacter cloacae Complex Isolates. Frontiers in Microbiology 10 (2019) 2582. https://doi.org/10.3389/fmicb.2019.02582
D. Faroongsarng. Theoretical Aspects of Differential Scanning Calorimetry as a Tool for the Studies of Equilibrium Thermodynamics in Pharmaceutical Solid Phase Transitions. AAPS PharmSciTech 17 (2016) 572-577. https://doi.org/10.1208/s12249-016-0530-2
A.V.P. Bobadilla, J. Arévalo, E. Sarró, H.M. Byrne, P.K. Maini, T. Carraro, S. Balocco, A. Meseguer, T. Alarcón. In vitro cell migration quantification method for scratch assays. Journal of the Royal Society, Interface 16 (2019) 20180709. https://doi.org/10.1098/rsif.2018.0709
G. Arya, M. Das, S.K. Sahoo. Evaluation of curcumin loaded chitosan/PEG blended PLGA nanoparticles for effective treatment of pancreatic cancer. Biomedicine and Pharmacotherapy 102 (2018) 555-566. https://doi.org/10.1016/j.biopha.2018.03.101
W. Arozal, M. Louisa, D. Rahmat, P. Chendrana, N.M.D. Sandhiutami. Development, Characterization and Pharmacokinetic Profile of Chitosan-Sodium Tripolyphosphate Nanoparticles Based Drug Delivery Systems for Curcumin. Advanced Pharmaceutical Bulletin 11 (2021) 77-85. https://doi.org/10.34172/apb.2021.008
M.A. Abdel-Hakeem, S. Mongy, B. Hassan, O.I. Tantawi, I. Badawy. Curcumin Loaded Chitosan-Protamine Nanoparticles Revealed Antitumor Activity Via Suppression of NF-κB, Proinflammatory Cytokines and Bcl-2 Gene Expression in the Breast Cancer Cells. Journal of Pharmaceutical Sciences 110 (2021) 3298-3305. https://doi.org/10.1016/j.xphs.2021.06.004
S. Bhunchu, C. Muangnoi, P. Rojsitthisak, P. Rojsitthisak. Curcumin diethyl disuccinate encapsulated in chitosan/alginate nanoparticles for improvement of its in vitro cytotoxicity against MDA-MB-231 human breast cancer cells. Pharmazie 71 (2016) 691-700. https://doi.org/10.1691/ph.2016.6105
M. Mobaraki, D. Bizari, M. Soltani, H. Khshmohabat, K. Raahemifar, M. Akbarzade Amirdehi. The Effects of Curcumin Nanoparticles Incorporated into Collagen-Alginate Scaffold on Wound Healing of Skin Tissue in Trauma Patients. Polymers (Basel) 13 (2021) 4291. https://doi.org/10.3390/polym13244291
Downloads
Published
How to Cite
Issue
Section
License
Articles are published under the terms and conditions of the
Creative Commons Attribution license 4.0 International.
