Electrochemical determination of an antitumour platinum(IV) complex: trans-[PtCl2(OH)2(dimethylamine)(isopropylamine)]. Application to biological samples

  • Carmen S. Hernandez Domínguez Departamento de Química Analítica y Análisis Instrumental, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, 28029 Madrid
  • Pedro Hernández Departamento de Química Analítica y Análisis Instrumental, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, 28029 Madrid

Abstract

A differential pulse voltammetry (DPV) method has been applied for the first time for de­termination of trans-Pt[Cl2(OH)2(dimethylamine)(isopropylamine)]. To this end, all che­mical and instrumental variables affecting the determination of trans-Pt[Cl2(OH)2(di­methylamine)(isopropylamine)] were optimized. From studies of the mechanisms go­verning the electrochemical response of trans-Pt[Cl2(OH)2(dimethyl­amine)(iso­pro­pyl­amine)], it was concluded that it is an electrochemically irreversible system with a reduc­tion under diffusion control, with a reduction potential of -425 mV. Under optimal condi­tions, the variation in the analytical signal (Ip) with trans-Pt[Cl2(OH)2(di­me­thyl­ami­ne)(iso­pro­pylamine)] concentration is linear in the 0.8 µg mL-1 to 20 µg mL-1 range, with an LOD of 97 ng mL-1 and a LOQ of 323 ng mL-1, RSD = 1.58 % and Er = 0.83 %. The optimized method was applied to the determination of trans-Pt[Cl2(OH)2(di­methyl­ami­ne)(iso­pro­pyl­amine)] in biological fluids, human urine and synthetic urine.

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Published
19-04-2013
Section
Bioelectrochemistry & Fuel Cells