The interactions between lipase and pyridinium ligands investigated by electrochemical and spectrophotometric methods

Authors

  • Simona Patriche Department of Chemistry, Physics and Environment, Faculty of Sciences and Environment, “Dunărea de Jos” University of Galati, 111 Domneasca Street, 800201 Galati
  • Elena Georgiana Lupu Department of Chemistry, Physics and Environment, Faculty of Sciences and Environment, “Dunărea de Jos” University of Galati, 111 Domneasca Street, 800201 Galati
  • Andreea Cârâc Department of Fundamental Science, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy of Bucharest, 6 Train Vuia Street, 020956 Bucharest
  • Rodica Mihaela Dinică Department of Chemistry, Physics and Environment, Faculty of Sciences and Environment, “Dunărea de Jos” University of Galati, 111 Domneasca Street, 800201 Galati
  • Geta Cârâc Department of Chemistry, Physics and Environment, Faculty of Sciences and Environment, “Dunărea de Jos” University of Galati, 111 Domneasca Street, 800201 Galati

DOI:

https://doi.org/10.5599/jese.232

Keywords:

Enzyme, Cyclic voltammetry, Morphology, Physicochemical properties, Pyridine

Abstract

The interaction between pyridinium ligands derived from 4,4’-bipyridine (N,N’-bis(p-bromophenacyl)-4,4’-bipyridinium dibromide – Lr) and (N,N’-bis(p-bromophenacyl)-1,2-bis (4-pyridyl) ethane dibromide – Lm) with lipase enzyme was evaluated. The stability of the pyridinium ligands, having an essential role in biological systems, in 0.1 M KNO3 as supporting electrolyte is influenced by the lipase concentration added. The pH and conductometry measurements in aqueous solution suggest a rapid ionic exchange process. The behavior of pyridinium ligands in the presence of lipase is investigated by cyclic voltammetry and UV/Vis spectroscopy, which indicated bindings and changes from the interaction between them. The voltammograms recorded on the glassy carbon electrode showed a more intense electronic transfer for the Lr interaction with lipase compared to Lm, which is due to the absence of mobile ethylene groups from Lr structure.

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Published

21-04-2016

How to Cite

Patriche, S., Lupu, E. G., Cârâc, A., Dinică, R. M., & Cârâc, G. (2016). The interactions between lipase and pyridinium ligands investigated by electrochemical and spectrophotometric methods. Journal of Electrochemical Science and Engineering, 6(1), 91–104. https://doi.org/10.5599/jese.232

Issue

Section

Electrochemical Science